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ProductsBack/TARC antibody [9K49]

TARC antibody [9K49]

GTX53261
GeneTex
ApplicationsWestern Blot, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetCcl17
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Overview

  • Supplier
    GeneTex
  • Product Name
    TARC antibody [9K49]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9K49
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID20295
  • Target name
    Ccl17
  • Target description
    C-C motif chemokine ligand 17
  • Target synonyms
    Abcd-2, Scya17, Scya17l, Tarc, C-C motif chemokine 17, CC chemokine TARC, chemokine (C-C motif) ligand 17, small inducible cytokine subfamily A17, small-inducible cytokine A17, thymus and activation-regulated chemokine
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDQ9WUZ6
  • Protein Name
    C-C motif chemokine 17
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TARC using anti-TARC antibody (PB9031). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Mouse TARC Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARC antigen affinity purified polyclonal antibody (Catalog # PB9031) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TARC at approximately 34KD. The expected band size for TARC is at 34KD.
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