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ProductsBack/TARS2 antibody [N3C2], Internal

TARS2 antibody [N3C2], Internal

GTX120121
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTARS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TARS2 antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.83 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80222
  • Target name
    TARS2
  • Target description
    threonyl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    COXPD21, TARSL1, thrRS, threonine--tRNA ligase, mitochondrial, threonyl-tRNA synthetase 2, mitochondrial (putative), threonyl-tRNA synthetase-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BW92
  • Protein Name
    Threonine--tRNA ligase, mitochondrial
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Diodato D, Melchionda L, Haack TB, et al. VARS2 and TARS2 mutations in patients with mitochondrial encephalomyopathies. Hum Mutat. 2014,35(8):983-9. doi: 10.1002/humu.22590
    Read this paper

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Figure 1. Western blot analysis of TARS2 using anti-TARS2 antibody (A12437). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARS2 antigen affinity purified polyclonal antibody (Catalog # A12437) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TARS2 at approximately 72 kDa. The expected band size for TARS2 is at 72 kDa.
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