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TAS2R10 Antibody

CSB-PA020245
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTAS2R10
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAS2R10 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID50839
  • Target name
    TAS2R10
  • Target description
    taste 2 receptor member 10
  • Target synonyms
    T2R10, TRB2, taste receptor type 2 member 10, taste receptor, family B, member 2, taste receptor, type 2, member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYW0
  • Protein Name
    Taste receptor type 2 member 10
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TAS2R10 using anti-TAS2R10 antibody (A10959-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS2R10 antigen affinity purified polyclonal antibody (Catalog # A10959-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAS2R10 at approximately 38 kDa. The expected band size for TAS2R10 is at 35 kDa.
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