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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate All lanes: TAT antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate All lanes: TAT antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate All lanes: TAT antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa

TAT Antibody

CSB-PA023175LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetTAT
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAT Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6898
  • Target name
    TAT
  • Target description
    tyrosine aminotransferase
  • Target synonyms
    L-tyrosine:2-oxoglutarate aminotransferase; testis tissue sperm-binding protein Li 34a; tyrosine aminotransferase; tyrosine aminotransferase, cytosolic
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17735
  • Protein Name
    Tyrosine aminotransferase
  • Scientific Description
    Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATTY using anti-ATTY antibody (A00622). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATTY antigen affinity purified polyclonal antibody (Catalog # A00622) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATTY at approximately 42KD. The expected band size for ATTY is at 50KD.
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