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TBCA antibody

GTX130832
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTBCA
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Overview

  • Supplier
    GeneTex
  • Product Name
    TBCA antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6902
  • Target name
    TBCA
  • Target description
    tubulin folding cofactor A
  • Target synonyms
    CFA; chaperonin cofactor A; epididymis secretory sperm binding protein; TCP1-chaperonin cofactor A; tubulin-specific chaperone A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75347
  • Protein Name
    Tubulin-specific chaperone A
  • Scientific Description
    The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CFA/TBCA using anti-CFA/TBCA antibody (A06926-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFA/TBCA antigen affinity purified polyclonal antibody (Catalog # A06926-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFA/TBCA at approximately 18 kDa. The expected band size for CFA/TBCA is at 18 kDa.
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