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ProductsBack/TBR1 Antibody - middle region (ARP31497_P050)

TBR1 Antibody - middle region (ARP31497_P050)

ARP31497_P050
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine
TargetTBR1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    TBR1 Antibody - middle region (ARP31497_P050)
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID10716
  • Target name
    TBR1
  • Target description
    T-box brain transcription factor 1
  • Target synonyms
    AUTS5, IDDAS, TBR-1, TES-56, T-box brain protein 1, T-box, brain 1, T-brain-1
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TBR1 using anti-TBR1 antibody (A07503-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBR1 antigen affinity purified polyclonal antibody (Catalog # A07503-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBR1 at approximately 74 kDa. The expected band size for TBR1 is at 74 kDa.
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