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Tbx21 Polyclonal Antibody

BS-3599R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTBX21
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Overview

  • Supplier
    Bioss
  • Product Name
    Tbx21 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID30009
  • Target name
    TBX21
  • Target description
    T-box transcription factor 21
  • Target synonyms
    IMD88, T-PET, T-bet, TBET, TBLYM, T-box transcription factor TBX21, T-box 21, T-box expressed in T cells, T-box protein 21, T-box transcription factor expressed in T cells, T-cell-specific T-box transcription factor T-bet, transcription factor TBLYM
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Lee SY, Bae CS, Seo JH, et al. Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model. Mol Med Rep. 2018,17(1):11-20. doi: 10.3892/mmr.2017.7901
    Read this paper
  • Chen PC, Hsieh MH, Kuo WS, et al. Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis. J Allergy Clin Immunol. 2017,140(4):1146-1149.e8. doi: 10.1016/j.jaci.2017.03.022
    Read this paper
  • Seo JH, Bang MA, Kim G, et al. Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma. Int J Mol Med. 2016,37(5):1221-8. doi: 10.3892/ijmm.2016.2541
    Read this paper

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Figure 1. Western blot analysis of TBX21 using anti-TBX21 antibody (A00404-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX21 antigen affinity purified polyclonal antibody (Catalog # A00404-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBX21 at approximately 65KD. The expected band size for TBX21 is at 58KD.
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