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ProductsBack/TCF12 / HEB Antibody

TCF12 / HEB Antibody

LS-C668057
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTCF12
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TCF12 / HEB Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of TCF12 expression in SKOV3 (A), A549 (B), mouse brain (C), rat testis (D) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of TCF12 expression in SKOV3 (A), A549 (B), mouse brain (C), rat testis (D) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6938
  • Target name
    TCF12
  • Target description
    transcription factor 12
  • Target synonyms
    bHLHb20; class B basic helix-loop-helix protein 20; CRS3; DNA-binding protein HTF4; E-box-binding protein; HEB; helix-loop-helix transcription factor 4; HsT17266; HTF4; p64; TCF-12; transcription factor 12; transcription factor HTF-4
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HEB/TCF12 using anti-HEB/TCF12 antibody (A02592-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEB/TCF12 antigen affinity purified polyclonal antibody (Catalog # A02592-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEB/TCF12 at approximately 85 kDa. The expected band size for HEB/TCF12 is at 73 kDa.
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