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ProductsBack/TCF7L1 / TCF-3 Antibody (290-370 aa, Internal)

TCF7L1 / TCF-3 Antibody (290-370 aa, Internal)

LS-C386343
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTCF7L1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TCF7L1 / TCF-3 Antibody (290-370 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID83439
  • Target name
    TCF7L1
  • Target description
    transcription factor 7 like 1
  • Target synonyms
    transcription factor 7-like 1, HMG box transcription factor 3, transcription factor 7-like 1 (T-cell specific, HMG-box)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TCF7L1 using anti-TCF7L1 antibody (PB9897). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF7L1 antigen affinity purified polyclonal antibody (Catalog # PB9897) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCF7L1 at approximately 63 kDa. The expected band size for TCF7L1 is at 63 kDa.
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