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ELISA analysis of antigen using GTX60505 TCP1 beta antibody [5B5C4]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
ELISA analysis of antigen using GTX60505 TCP1 beta antibody [5B5C4]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
ELISA analysis of antigen using GTX60505 TCP1 beta antibody [5B5C4]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng

TCP1 beta antibody [5B5C4]

GTX60505
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetCCT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TCP1 beta antibody [5B5C4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B5C4
  • Conjugate
    Unconjugated
  • Gene ID10576
  • Target name
    CCT2
  • Target description
    chaperonin containing TCP1 subunit 2
  • Target synonyms
    99D8.1; CCTB; CCT-beta; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); epididymis secretory sperm binding protein Li 100n; HEL-S-100n; PRO1633; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; TCP-1-beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP78371
  • Protein Name
    T-complex protein 1 subunit beta
  • Scientific Description
    The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCT2 using anti-CCT2 antibody (PB9992). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human HEL whole cell lysates, Lane 7: human Raji whole cell lysates, Lane 8: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (Catalog # PB9992) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT2 at approximately 57 kDa. The expected band size for CCT2 is at 57 kDa.
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Western blot All lanes: CCT2 antibody at 5.63ug/ml Lane 1: Jurkat whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 53 kDa Observed band size: 58 kDa
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