Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with testis antigen 1B Polyclonal Antibody, Unconjugated bs-4501R at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.