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ProductsBack/TF / Transferrin Antibody (clone 9B3)

TF / Transferrin Antibody (clone 9B3)

LS-C771365
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TF / Transferrin Antibody (clone 9B3)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:100 - 1:300), IHC-P, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9B3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7018
  • Target name
    TF
  • Target description
    transferrin
  • Target synonyms
    HEL-S-71p, PRO1557, PRO2086, TFQTL1, serotransferrin, beta-1 metal-binding globulin, epididymis secretory sperm binding protein Li 71p, siderophilin
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Transferrin using anti-Transferrin antibody (PB9827). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse Hepa1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Transferrin antigen affinity purified polyclonal antibody (Catalog # PB9827) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Transferrin at approximately 77 kDa. The expected band size for Transferrin is at 77 kDa.
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Lane 1: Human Serum probed with Transferrin Polyclonal Antibody, Unconjugated (bs-2052R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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