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WB analysis of various sample lysates using GTX33541 TFEB antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33541 TFEB antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33541 TFEB antibody. Dilution : 1:1000 Loading : 25microg per lane

TFEB antibody

GTX33541
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTFEB
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Overview

  • Supplier
    GeneTex
  • Product Name
    TFEB antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7942
  • Target name
    TFEB
  • Target description
    transcription factor EB
  • Target synonyms
    ALPHATFEB; BHLHE35; class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; transcription factor EB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19484
  • Protein Name
    Transcription factor EB
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Restoration of ER proteostasis attenuates remote apoptotic cell death after spinal cord injury by reducing autophagosome overload. Bisicchia E et al., 2022 Apr 20, Cell Death Dis
    Read more
  • Genetic and chemical activation of TFEB mediates clearance of aggregated alpha-synuclein. Kilpatrick K et al., 2015, PLoS One
    Read more

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Figure 1. Western blot analysis of TFEB using anti-TFEB antibody (A00662-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody (Catalog # A00662-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFEB at approximately 74KD. The expected band size for TFEB is at 74KD.
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