Various whole cell extracts (30 Aμg) were separated by 5% SDS-PAGE, and the membrane was blotted with TFII-I antibody [HL1674] (GTX637273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TFII-I antibody [HL1674] (GTX637273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637273/GTX637273_T-44760_20221014_WB_shRNA_watermark_22101717_269.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TFII-I antibody [HL1674] (GTX637273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![TFII-I antibody [HL1674] detects TFII-I protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFII-I stained by TFII-I antibody [HL1674] (GTX637273) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637273/GTX637273_T-44760_20220916_ICC_IF_22102723_434.webp "TFII-I antibody [HL1674] detects TFII-I protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFII-I stained by TFII-I antibody [HL1674] (GTX637273) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
Various whole cell extracts (30 Aμg) were separated by 5% SDS-PAGE, and the membrane was blotted with TFII-I antibody [HL1674] (GTX637273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
TFII-I antibody [HL1674]
GTX637273
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetGTF2I
Overview
- SupplierGeneTex
- Product NameTFII-I antibody [HL1674]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1674
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2969
- Target nameGTF2I
- Target descriptiongeneral transcription factor IIi
- Target synonymsBAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6, general transcription factor II-I, BTK-associated protein, 135kD, Bruton tyrosine kinase-associated protein 135, SRF-Phox1-interacting protein, Williams-Beuren syndrome chromosome region 6
- HostRabbit
- IsotypeIgG
- Protein IDP78347
- Protein NameGeneral transcription factor II-I
- Scientific DescriptionThis gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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