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ProductsBack/TFPI / LACI Antibody (Preservative Free)

TFPI / LACI Antibody (Preservative Free)

LS-C818322
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetTFPI
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TFPI / LACI Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ELISA
  • Applications Supplier
    ELISA (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7035
  • Target name
    TFPI
  • Target description
    tissue factor pathway inhibitor
  • Target synonyms
    EPI, LACI, TFI, TFPI1, tissue factor pathway inhibitor, anti-convertin, extrinsic pathway inhibitor, tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TFPI using anti-TFPI antibody (A01052). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFPI antigen affinity purified polyclonal antibody (Catalog # A01052) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFPI at approximately 45KD. The expected band size for TFPI is at 35KD.
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