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Western Blot analysis of H460 293 cells using TFPI-2 Polyclonal Antibody
Western Blot analysis of H460 293 cells using TFPI-2 Polyclonal Antibody
Western Blot analysis of H460 293 cells using TFPI-2 Polyclonal Antibody

TFPI2 Antibody

CSB-PA004987
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTFPI2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TFPI2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7980
  • Target name
    TFPI2
  • Target description
    tissue factor pathway inhibitor 2
  • Target synonyms
    placental protein 5; PP5; REF1; retinal pigment epithelium cell factor 1; TFPI-2; tissue factor pathway inhibitor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48307
  • Protein Name
    Tissue factor pathway inhibitor 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TFPI2 using anti-TFPI2 antibody (A03697-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: HELA whole Cell lysates, Lane 3: human placenta tissue lysates, Lane 4: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFPI2 antigen affinity purified polyclonal antibody (Catalog # A03697-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFPI2 at approximately 27KD, 35KD. The expected band size for TFPI2 is at 27KD.
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