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ProductsBack/TGFalpha(1E8-G6), 0.2mg/mL [26628-22-8]
TGFalpha(1E8-G6), 0.2mg/mL [26628-22-8]

TGFalpha(1E8-G6), 0.2mg/mL [26628-22-8]

BNUB0785
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rabbit, Zebra Fish
TargetNCAM1
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Overview

  • Supplier
    Biotium
  • Product Name
    TGFalpha(1E8-G6), 0.2mg/mL [26628-22-8]
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • CAS Number
    26628-22-8
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1E8-G6
  • Concentration
    0.2 mg/ml
  • Gene ID4684
  • Target name
    NCAM1
  • Target description
    neural cell adhesion molecule 1
  • Target synonyms
    CD56, MSK39, NCAM, neural cell adhesion molecule 1, antigen recognized by monoclonal antibody 5.1H11, neural cell adhesion molecule, NCAM
  • Host
    Mouse
  • Isotype
    IgM, IgG1
  • Protein IDP01135
  • Protein Name
    Protransforming growth factor alpha
  • Scientific Description
    This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.
  • Source
    Animal
  • Reactivity
    Bovine, Human, Mouse, Rabbit, Zebra Fish
  • Storage Instruction
    2°C to 8°C,RT
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of NCAM1 using anti-NCAM1 antibody (A00184-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 antigen affinity purified polyclonal antibody (Catalog # A00184-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAM1 at approximately 150KD. The expected band size for NCAM1 is at 150KD.
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