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Western Blot analysis of NIH-3T3 cells using TGFbeta RII Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using TGFbeta RII Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using TGFbeta RII Polyclonal Antibody

TGFBR2 Antibody

CSB-PA004277
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTGFBR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TGFBR2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7048
  • Target name
    TGFBR2
  • Target description
    transforming growth factor beta receptor 2
  • Target synonyms
    AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II, TGF-beta receptor type-2, TGF-beta receptor type IIB, TGF-beta type II receptor, transforming growth factor beta receptor II, transforming growth factor beta receptor type IIC, transforming growth factor, beta receptor II (70/80kDa), transforming growth factor, beta receptor II alpha, transforming growth factor, beta receptor II beta, transforming growth factor, beta receptor II delta, transforming growth factor, beta receptor II epsilon, transforming growth factor, beta receptor II gamma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP37173
  • Protein Name
    TGF-beta receptor type-2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TGFBR2 using anti-TGFBR2 antibody (PB9829). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFBR2 antigen affinity purified polyclonal antibody (Catalog # PB9829) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFBR2 at approximately 70 kDa. The expected band size for TGFBR2 is at 65 kDa.
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