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TGM2 Antibody (OAAB19793)

OAAB19793
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTGM2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    TGM2 Antibody (OAAB19793)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
  • Gene ID7052
  • Target name
    TGM2
  • Target description
    transglutaminase 2
  • Target synonyms
    C polypeptide, protein-glutamine-gamma-glutamyltransferase; erythrocyte transglutaminase; G(h); heart G alpha(h); hhG alpha(h); hTG2; isopeptidase TGM2; protein G alpha(h); protein-glutamine deamidase TGM2; protein-glutamine dopaminyltransferase TGM2; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine histaminyltransferase TGM2; protein-glutamine noradrenalinyltransferase TGM2; protein-glutamine serotonyltransferase TGM2; TG(C); TGase C; TGase H; TGase II; TGase-2; TGC; tissue transglutaminase; transglutaminase C; transglutaminase H; transglutaminase II; tTG; tTgase
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TGM2 using anti-TGM2 antibody (A02229). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGM2 antigen affinity purified polyclonal antibody (Catalog # A02229) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGM2 at approximately 80 kDa. The expected band size for TGM2 is at 80 kDa.
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