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THAP11 Antibody

CSB-PA846612LA01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetTHAP11
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Overview

  • Supplier
    Cusabio
  • Product Name
    THAP11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57215
  • Target name
    THAP11
  • Target description
    THAP domain containing 11
  • Target synonyms
    CTG-B43a; CTG-B45d; HRIHFB2206; RONIN; THAP domain-containing protein 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EK4
  • Protein Name
    THAP domain-containing protein 11
  • Scientific Description
    Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: A549 Cell lysates; Lane 2: NIH/3T3 Cell lysates; probed with THAP11 (364CT25.4.2) Monoclonal Antibody, unconjugated (bsm-51114M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Figure 1. Western blot analysis of THAP11 using anti-THAP11 antibody (A08519-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THAP11 antigen affinity purified polyclonal antibody (Catalog # A08519-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THAP11 at approximately 50 kDa. The expected band size for THAP11 is at 34 kDa.
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