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ProductsBack/THBS1 / Thrombospondin-1 Antibody (aa58-86)

THBS1 / Thrombospondin-1 Antibody (aa58-86)

LS-C407663
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Equine, Human, Mammals, Porcine
TargetTHBS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    THBS1 / Thrombospondin-1 Antibody (aa58-86)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7057
  • Target name
    THBS1
  • Target description
    thrombospondin 1
  • Target synonyms
    THBS, THBS-1, TSP, TSP-1, TSP1, thrombospondin-1, glycoprotein G, thrombospondin-1p180, thrombospondin-p50
  • Host
    Rabbit
  • Reactivity
    Canine, Equine, Human, Mammals, Porcine
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Thrombospondin using anti-Thrombospondin antibody (A00667-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin antigen affinity purified polyclonal antibody (Catalog # A00667-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombospondin at approximately 165KD, 180KD. The expected band size for Thrombospondin is at 130KD.
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