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WB analysis of human liver tissue lysate using GTX31383 THEM4 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human liver tissue lysate using GTX31383 THEM4 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human liver tissue lysate using GTX31383 THEM4 antibody. Working concentration : (A) 1 and (B) 2 microg/ml

THEM4 antibody

GTX31383
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTHEM4
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Overview

  • Supplier
    GeneTex
  • Product Name
    THEM4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 2.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID117145
  • Target name
    THEM4
  • Target description
    thioesterase superfamily member 4
  • Target synonyms
    acyl-CoA thioesterase THEM4; acyl-coenzyme A thioesterase THEM4; carboxyl-terminal modulator protein; C-terminal modulator protein; CTMP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T1C6
  • Protein Name
    Acyl-coenzyme A thioesterase THEM4
  • Scientific Description
    Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of THEM4 using anti-THEM4 antibody (A07814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THEM4 antigen affinity purified polyclonal antibody (Catalog # A07814-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THEM4 at approximately 22 kDa. The expected band size for THEM4 is at 27 kDa.
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