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ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00795 TIE2 (phospho Tyr897) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00795 TIE2 (phospho Tyr897) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00795 TIE2 (phospho Tyr897) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml

TIE2 (phospho Tyr897) antibody

GTX00795
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTEK
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Overview

  • Supplier
    GeneTex
  • Product Name
    TIE2 (phospho Tyr897) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7010
  • Target name
    TEK
  • Target description
    TEK receptor tyrosine kinase
  • Target synonyms
    angiopoietin-1 receptor; CD202B; endothelial tyrosine kinase; GLC3E; TEK tyrosine kinase, endothelial; TIE2; TIE-2; tunica interna endothelial cell kinase; tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protein kinase receptor TEK; tyrosine-protein kinase receptor TIE-2; VMCM; VMCM1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02763
  • Protein Name
    Angiopoietin-1 receptor
  • Scientific Description
    This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TEK using anti-TEK antibody (A01274-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEK antigen affinity purified polyclonal antibody (Catalog # A01274-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEK at approximately 160KD. The expected band size for TEK is at 126KD.
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