Bio-Connect
WB analysis of samples using GTX15826 TIF1 gamma antibody [16G9].
WB analysis of samples using GTX15826 TIF1 gamma antibody [16G9].
WB analysis of samples using GTX15826 TIF1 gamma antibody [16G9].

TIF1 gamma antibody [16G9]

GTX15826
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetTRIM33
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    TIF1 gamma antibody [16G9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 2 microg/mL. ICC/IF: 4 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    16G9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51592
  • Target name
    TRIM33
  • Target description
    tripartite motif containing 33
  • Target synonyms
    E3 ubiquitin-protein ligase TRIM33; ECTO; ectodermin homolog; protein Rfg7; PTC7; RET-fused gene 7 protein; RFG7; RING-type E3 ubiquitin transferase TRIM33; TF1G; TIF1G; TIF1GAMMA; TIF1-gamma; TIFGAMMA; transcriptional intermediary factor 1 gamma
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDQ9UPN9
  • Protein Name
    E3 ubiquitin-protein ligase TRIM33
  • Scientific Description
    The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

IHC-P analysis of human liver tissue using GTX31545 TIF1 gamma antibody. Working concentration : 2.5 microg/ml
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTRIM33
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetTRIM33
  • SizePrice
Antigen: bs-3582P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTRIM33
  • SizePrice
Anti-TRIM33 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA104649(TRIM33 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
TRIM33 AntibodyCSB-PA104649
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetTRIM33
  • SizePrice
Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA004345 antibody. Corresponding TRIM33 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTRIM33
  • SizePrice
Figure 1. Western blot analysis of TIF1 gamma using anti-TIF1 gamma antibody (PB9836). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIF1 gamma antigen affinity purified polyclonal antibody (Catalog # PB9836) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIF1 gamma at approximately 150 kDa. The expected band size for TIF1 gamma is at 123 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTRIM33
  • SizePrice
IHC-P analysis of human breast adenocarcinoma tissue using GTX83492 TIF1 gamma antibody [9D4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTRIM33
  • SizePrice
IHC-P analysis of human breast adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTRIM33
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTRIM33
  • SizePrice