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TIMD4 Antibody (YA4427, Mouse)

HY-P84730
MedChem Express
TargetTIMD4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TIMD4 Antibody (YA4427, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID91937
  • Target name
    TIMD4
  • Target description
    T cell immunoglobulin and mucin domain containing 4
  • Target synonyms
    SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4, TIMD-4
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    TIMD4 Antibody (YA4427) is a mouse-derived and non-conjugated IgG1 antibody, targeting to TIMD4. It can be applicated for ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161
  • Species
    Mouse

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Figure 1. Western blot analysis of TIM 4/TIMD4 using anti-TIM 4/TIMD4 antibody (A06637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 4/TIMD4 antigen affinity purified polyclonal antibody (Catalog # A06637-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 4/TIMD4 at approximately 42 kDa. The expected band size for TIM 4/TIMD4 is at 42 kDa.
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