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Western blot analysis of extracts of various cell lines using TIMM17A Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using TIMM17A Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using TIMM17A Polyclonal Antibody at 1:1000 dilution.

TIMM17A Polyclonal Antibody

E-AB-64730
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TIMM17A Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIMM17A using anti-TIMM17A antibody (A12168-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse Neuro-2a whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMM17A antigen affinity purified polyclonal antibody (Catalog # A12168-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMM17A at approximately 18KD. The expected band size for TIMM17A is at 18KD.
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