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TIMP-3 Polyclonal Antibody

BS-0417R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTIMP3
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Overview

  • Supplier
    Bioss
  • Product Name
    TIMP-3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7078
  • Target name
    TIMP3
  • Target description
    TIMP metallopeptidase inhibitor 3
  • Target synonyms
    HSMRK222, K222, K222TA2, SFD, metalloproteinase inhibitor 3, MIG-5 protein, TIMP-3, protein MIG-5, tissue inhibitor of metalloproteinases 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35625
  • Protein Name
    Metalloproteinase inhibitor 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Han J, Liang H, Yi J, et al. Long-Term Selenium-Deficient Diet Induces Liver Damage by Altering Hepatocyte Ultrastructure and MMP1/3 and TIMP1/3 Expression in Growing Rats. Biol Trace Elem Res. 2017,175(2):396-404. doi: 10.1007/s12011-016-0781-5
    Read this paper
  • Lu M, Cao J, Liu F, et al. The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage. Cells Tissues Organs. 2012,196(3):241-50. doi: 10.1159/000335046
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TIMP3 using anti-TIMP3 antibody (A00477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody (Catalog # A00477) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP3 at approximately 24KD. The expected band size for TIMP3 is at 24KD.
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