ChIP analysis of HEK293T and NIH3T3 cells using GTX60851 TIP-5 antibody - ChIP grade. Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4?C with 100 microg sheared chromatin and either 5 microl of the TIP5 antibody or 5 microl IgG which was used as negative IP control. The IPd DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. This figure shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.