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WB analysis of various samples using GTX12276 TJP2 antibody. Lane 1 : rat brain tissue lysate Lane 2 : PC-12 cell lysate Lane 3 : mouse brain tissue lysate Lane 4 : HEPA cell lysate
WB analysis of various samples using GTX12276 TJP2 antibody. Lane 1 : rat brain tissue lysate Lane 2 : PC-12 cell lysate Lane 3 : mouse brain tissue lysate Lane 4 : HEPA cell lysate
WB analysis of various samples using GTX12276 TJP2 antibody. Lane 1 : rat brain tissue lysate Lane 2 : PC-12 cell lysate Lane 3 : mouse brain tissue lysate Lane 4 : HEPA cell lysate

ZO-2 antibody

GTX12276
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetTjp2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZO-2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. IHC-P: 0.5-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID21873
  • Target name
    Tjp2
  • Target description
    tight junction protein 2
  • Target synonyms
    ZO-2, tight junction protein 2, tight junction protein ZO-2, Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2), zona occludens protein 2, zonula occludens protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Z0U1
  • Protein Name
    Tight junction protein 2
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TJP2 using anti-TJP2 antibody (PA1971). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat RH35 whole cell lysates, Lane 9: rat HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP2 antigen affinity purified polyclonal antibody (Catalog # PA1971) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP2 at approximately 150 kDa. The expected band size for TJP2 is at 131 kDa.
Product group Antibodies
Boster Bio
Anti-Tight junction protein ZO-2 TJP2 Antibody Picoband(r)PA1971-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetTjp2
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