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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA023577ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA023577ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA023577ESR2HU at dilution of 1:100

TK1 Antibody

CSB-PA023577ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7083
  • Target name
    TK1
  • Target description
    thymidine kinase 1
  • Target synonyms
    cytosolic thymidine kinase; epididymis secretory sperm binding protein; thymidine kinase, cytosolic; TK2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04183
  • Protein Name
    Thymidine kinase, cytosolic
  • Scientific Description
    TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TK1 using anti-TK1 antibody (PB9832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: SMMC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TK1 antigen affinity purified polyclonal antibody (Catalog # PB9832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TK1 at approximately 25 kDa. The expected band size for TK1 is at 25 kDa.
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