TLE1 antibody [N2C1], Internal detects TLE1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLE1 stained by TLE1 antibody [N2C1], Internal (GTX110092) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
![Wild-type (WT) and TLE1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLE1 antibody [N2C1], Internal (GTX110092) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110092/GTX110092_40058_20170622_WB_KO_watermark_w_23060500_727.webp "Wild-type (WT) and TLE1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLE1 antibody [N2C1], Internal (GTX110092) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
TLE1 antibody [N2C1], Internal detects TLE1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLE1 stained by TLE1 antibody [N2C1], Internal (GTX110092) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
TLE1 antibody [N2C1], Internal
GTX110092
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetTLE1
Overview
- SupplierGeneTex
- Product NameTLE1 antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.92 mg/ml
- ConjugateUnconjugated
- Gene ID7088
- Target nameTLE1
- Target descriptionTLE family member 1, transcriptional corepressor
- Target synonymsESG, ESG1, GRG1, TLE-1, transducin-like enhancer protein 1, enhancer of split groucho-like protein 1, transducin like enhancer of split 1, transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
- HostRabbit
- IsotypeIgG
- Protein IDQ04724
- Protein NameTransducin-like enhancer protein 1
- Scientific DescriptionTranscriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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