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Western blot All lanes: TLN2 antibody at 12microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 272 kDa Observed band size: 230 kDa
Western blot All lanes: TLN2 antibody at 12microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 272 kDa Observed band size: 230 kDa
Western blot All lanes: TLN2 antibody at 12microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 272 kDa Observed band size: 230 kDa

TLN2 Antibody

CSB-PA897282HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTLN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TLN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83660
  • Target name
    TLN2
  • Target description
    talin 2
  • Target synonyms
    ILWEQ, talin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4G6
  • Protein Name
    Talin-2
  • Scientific Description
    As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Talin 2 using anti-Talin 2 antibody (PB9961). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse cardiac muscle tissue lysates, Lane 3: SMMC7721 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Talin 2 antigen affinity purified polyclonal antibody (Catalog # PB9961) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Talin 2 at approximately 271 kDa. The expected band size for Talin 2 is at 272 kDa.
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