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Mouse spleen cells probed with \tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse spleen cells probed with \tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse spleen cells probed with \tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

TLR2 Polyclonal Antibody

BS-1019R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTLR2
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Overview

  • Supplier
    Bioss
  • Product Name
    TLR2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7097
  • Target name
    TLR2
  • Target description
    toll like receptor 2
  • Target synonyms
    CD282, TIL4, toll-like receptor 2, toll/interleukin-1 receptor-like protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60603
  • Protein Name
    Toll-like receptor 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhan Q, Wang Q, Lin R, et al. Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Int J Biol Macromol. 2020,145:1080-1090. doi: 10.1016/j.ijbiomac.2019.09.201
    Read this paper
  • Li T, Gao J, Zhao X, et al. Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis. R Soc Open Sci. 2019,6(7):181604. doi: 10.1098/rsos.181604
    Read this paper
  • Dong W, Zhu Q, Yang B, et al. Polychlorinated Biphenyl Quinone Induces Caspase 1-Mediated Pyroptosis through Induction of Pro-inflammatory HMGB1-TLR4-NLRP3-GSDMD Signal Axis. Chem Res Toxicol. 2019,32(6):1051-1057. doi: 10.1021/acs.chemrestox.8b00376
    Read this paper
  • Zhang J, Zhang ZG, Lu M, et al. MiR-146a promotes oligodendrocyte progenitor cell differentiation and enhances remyelination in a model of experimental autoimmune encephalomyelitis. Neurobiol Dis. 2019,125:154-162. doi: 10.1016/j.nbd.2019.01.019
    Read this paper
  • Mödinger Y, Rapp A, Pazmandi J, et al. C5aR1 interacts with TLR2 in osteoblasts and stimulates the osteoclast-inducing chemokine CXCL10. J Cell Mol Med. 2018,22(12):6002-6014. doi: 10.1111/jcmm.13873
    Read this paper
  • Barreiros D, Pucinelli CM, Oliveira KMH, et al. Immunohistochemical and mRNA expression of RANK, RANKL, OPG, TLR2 and MyD88 during apical periodontitis progression in mice. J Appl Oral Sci. 2018,26:e20170512. doi: 10.1590/1678-7757-2017-0512
    Read this paper
  • Xu W, Zheng D, Liu Y, et al. Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway. Cell Physiol Biochem. 2017,44(6):2091-2104. doi: 10.1159/000485947
    Read this paper
  • Schimunek L, Serve R, Teuben MPJ, et al. Early decreased TLR2 expression on monocytes is associated with their reduced phagocytic activity and impaired maturation in a porcine polytrauma model. PLoS One. 2017,12(11):e0187404. doi: 10.1371/journal.pone.0187404
    Read this paper
  • Wang JQ, Li XW, Liu M, et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma. Mol Med Rep. 2016,13(5):4289-302. doi: 10.3892/mmr.2016.5050
    Read this paper
  • Hardigan T, Abdul Y, Ergul A. Linagliptin reduces effects of ET-1 and TLR2-mediated cerebrovascular hyperreactivity in diabetes. Life Sci. 2016,159:90-96. doi: 10.1016/j.lfs.2016.02.067
    Read this paper

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Figure 1. Western blot analysis of TLR2 using anti-TLR2 antibody (A00131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 (-LPS) whole cell lysates, Lane 2: human THP-1 (+LPS) whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR2 antigen affinity purified polyclonal antibody (A00131) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR2 at approximately 105 kDa. The expected band size for TLR2 is at 90 kDa.
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