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TLR2 (TL2.1) HRP

SC-21759HRP
Santa Cruz Biotechnology
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTLR2
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    TLR2 (TL2.1) HRP
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    HRP
  • Gene ID7097
  • Target name
    TLR2
  • Target description
    toll like receptor 2
  • Target synonyms
    CD282, TIL4, toll-like receptor 2, toll/interleukin-1 receptor-like protein 4
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TLR2 using anti-TLR2 antibody (A00131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 (-LPS) whole cell lysates, Lane 2: human THP-1 (+LPS) whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR2 antigen affinity purified polyclonal antibody (A00131) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR2 at approximately 105 kDa. The expected band size for TLR2 is at 90 kDa.
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