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Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

TLR4 Polyclonal Antibody

BS-1021R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
TargetTlr4
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Overview

  • Supplier
    Bioss
  • Product Name
    TLR4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID21898
  • Target name
    Tlr4
  • Target description
    toll-like receptor 4
  • Target synonyms
    Lps, Ly87, Ran/M1, Rasl2-8, toll-like receptor 4, Toll like receptor 4, lipopolysaccharide response
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9QX05
  • Protein Name
    Toll-like receptor 4
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Nakayama Y, Isoyama N, Yamamoto T, et al. Receptor activator of the NFκB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Transpl Immunol. 2020,58:101263. doi: 10.1016/j.trim.2020.101263
    Read this paper
  • Winter J, Hammer E, Heger J, et al. Adenine Nucleotide Translocase 1 Expression is Coupled to the HSP27-Mediated TLR4 Signaling in Cardiomyocytes. Cells. 2019,8(12). doi: 10.3390/cells8121588
    Read this paper
  • Zhan Q, Wang Q, Lin R, et al. Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Int J Biol Macromol. 2020,145:1080-1090. doi: 10.1016/j.ijbiomac.2019.09.201
    Read this paper
  • Zhang CY, Ren XM, Li HB, et al. Simvastatin alleviates inflammation and oxidative stress in rats with cerebral hemorrhage through Nrf2-ARE signaling pathway. Eur Rev Med Pharmacol Sci. 2019,23(14):6321-6329. doi: 10.26355/eurrev_201907_18455
    Read this paper
  • Wang G, Xu B, Shi F, et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019,2019:7850324. doi: 10.1155/2019/7850324
    Read this paper
  • Han L, Li T, Du M, et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice. Nutrients. 2019,11(3). doi: 10.3390/nu11030670
    Read this paper
  • Li C, Li L, Chen K, et al. UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells. Oxid Med Cell Longev. 2019,2019:6505373. doi: 10.1155/2019/6505373
    Read this paper
  • Xu W, Zheng D, Liu Y, et al. Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway. Cell Physiol Biochem. 2017,44(6):2091-2104. doi: 10.1159/000485947
    Read this paper
  • Kulhankova K, Kinney KJ, Stach JM, et al. The Superantigen Toxic Shock Syndrome Toxin 1 Alters Human Aortic Endothelial Cell Function. Infect Immun. 2018,86(3). doi: 10.1128/IAI.00848-17
    Read this paper
  • Harasymowicz NS, Clement ND, Azfer A, et al. Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and Class III Obesity in Patients With Osteoarthritis. Arthritis Rheumatol. 2017,69(7):1396-1406. doi: 10.1002/art.40102
    Read this paper

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Figure 1. Western blot analysis of Tlr4 using anti-Tlr4 antibody (A00017-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr4 antigen affinity purified polyclonal antibody (Catalog # A00017-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr4 at approximately 96 kDa. The expected band size for Tlr4 is at 96 kDa.
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