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ProductsBack/TLR5 Polyclonal Antibody
Lane 1: Mouse Liver; Lane 2: Mouse Ovary; 40ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Liver; Lane 2: Mouse Ovary; 40ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Liver; Lane 2: Mouse Ovary; 40ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

TLR5 Polyclonal Antibody

BS-1197R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTlr5
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Overview

  • Supplier
    Bioss
  • Product Name
    TLR5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID53791
  • Target name
    Tlr5
  • Target description
    toll-like receptor 5
  • Target synonyms
    toll-like receptor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9JLF7
  • Protein Name
    Toll-like receptor 5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Shi D, Zhao S, Jiang W, et al. TLR5: A prognostic and monitoring indicator for triple-negative breast cancer. Cell Death Dis. 2019,10(12):954. doi: 10.1038/s41419-019-2187-8
    Read this paper
  • Li C, Yu Y, Li W, et al. Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway. Sci Rep. 2017,7(1):5843. doi: 10.1038/s41598-017-06021-5
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Tlr5 using anti-Tlr5 antibody (A00462-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr5 antigen affinity purified polyclonal antibody (Catalog # A00462-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr5 at approximately 100 kDa. The expected band size for Tlr5 is at 98 kDa.
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