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ProductsBack/TLR7 Mouse Monoclonal Antibody [Clone ID: 66H3]
IHC staining of human FFPE tonsil section (Vermi William courtesy)
IHC staining of human FFPE tonsil section (Vermi William courtesy)
IHC staining of human FFPE tonsil section (Vermi William courtesy)

TLR7 Mouse Monoclonal Antibody [Clone ID: 66H3]

DDX0500P-100
OriGene
ApplicationsFlow Cytometry, ImmunoHistoChemistry
Product group Antibodies
TargetTLR7
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TLR7 Mouse Monoclonal Antibody [Clone ID: 66H3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    66H3
  • Gene ID51284
  • Target name
    TLR7
  • Target description
    toll like receptor 7
  • Target synonyms
    IMD74; TLR7-like; toll-like receptor 7; toll-like receptor 7-like
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9NYK1
  • Protein Name
    Toll-like receptor 7
  • Scientific Description
    TLR7 mouse monoclonal antibody, clone 66H3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.
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