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ProductsBack/TLR7 Polyclonal Antibody
Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human 293T cell lysates probed with TLR7 Polyclonal Antibody, Unconjugated (bs-6601R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human 293T cell lysates probed with TLR7 Polyclonal Antibody, Unconjugated (bs-6601R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human 293T cell lysates probed with TLR7 Polyclonal Antibody, Unconjugated (bs-6601R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

TLR7 Polyclonal Antibody

BS-6601R
Bioss Antibodies
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetTLR7
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TLR7 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(WB=1:500-2000), ELISA(ELISA=1:5000-10000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID51284
  • Target name
    TLR7
  • Target description
    toll like receptor 7
  • Target synonyms
    IMD74; TLR7-like; toll-like receptor 7; toll-like receptor 7-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYK1
  • Protein Name
    Toll-like receptor 7
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos. Umehara T et al., 2020 Aug, Nat Protoc
    Read more
  • Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm. Umehara T et al., 2019 Aug, PLoS Biol
    Read more

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Figure 1. Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.
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