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Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti TLR9/CD289 Polyclonal Antibody, Unconjugated (bs-2717R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti TLR9/CD289 Polyclonal Antibody, Unconjugated (bs-2717R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti TLR9/CD289 Polyclonal Antibody, Unconjugated (bs-2717R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

TLR9 Polyclonal Antibody

BS-2717R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTLR9
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TLR9 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID54106
  • Target name
    TLR9
  • Target description
    toll like receptor 9
  • Target synonyms
    CD289; toll-like receptor 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR96
  • Protein Name
    Toll-like receptor 9
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Polychlorinated Biphenyl Quinone Induces Caspase 1-Mediated Pyroptosis through Induction of Pro-inflammatory HMGB1-TLR4-NLRP3-GSDMD Signal Axis. Dong W et al., 2019 Jun 17, Chem Res Toxicol
    Read more
  • From the Cover: Tetrachlorobenzoquinone Exerts Neurological Proinflammatory Activity by Promoting HMGB1 Release, Which Induces TLR4 Clustering within the Lipid Raft. Fu J et al., 2016 Oct, Toxicol Sci
    Read more

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Figure 1. Western blot analysis of TLR9 using anti-TLR9 antibody (A00198-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR9 antigen affinity purified polyclonal antibody (Catalog # A00198-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR9 at approximately 116 kDa. The expected band size for TLR9 is at 116 kDa.
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