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WB analysis of THP1 (A), NIH3T3 (B), H9C2 (C) whole cell lysates using GTX56016 TMC7 antibody.
WB analysis of THP1 (A), NIH3T3 (B), H9C2 (C) whole cell lysates using GTX56016 TMC7 antibody.
WB analysis of THP1 (A), NIH3T3 (B), H9C2 (C) whole cell lysates using GTX56016 TMC7 antibody.

TMC7 antibody

GTX56016
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTMC7
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Overview

  • Supplier
    GeneTex
  • Product Name
    TMC7 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79905
  • Target name
    TMC7
  • Target description
    transmembrane channel like 7
  • Target synonyms
    transmembrane channel-like protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z402
  • Protein Name
    Transmembrane channel-like protein 7
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMC7 using anti-TMC7 antibody (A14820-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMC7 antigen affinity purified polyclonal antibody (Catalog # A14820-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMC7 at approximately 90 kDa. The expected band size for TMC7 is at 84 kDa.
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