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TMEFF2 Antibody (clone OTI4G2)

LS-C173625
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetTMEFF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TMEFF2 Antibody (clone OTI4G2)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    Flo (1:100), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4G2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23671
  • Target name
    TMEFF2
  • Target description
    transmembrane protein with EGF like and two follistatin like domains 2
  • Target synonyms
    cancer/testis antigen family 120, member 2; CT120.2; HPP1; hyperplastic polyposis protein 1; TENB2; tomoregulin-2; TPEF; TR; TR-2; transmembrane protein TENB2; transmembrane protein with EGF-like and two follistatin-like domains
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMEFF2 using anti-TMEFF2 antibody (A03846-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEFF2 antigen affinity purified polyclonal antibody (Catalog # A03846-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEFF2 at approximately 41 kDa. The expected band size for TMEFF2 is at 41 kDa.
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Immunohistochemical staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal cells.
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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA451608(TMEFF2 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)
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