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ProductsBack/TMEM104 antibody, Internal
WB analysis of Jurkat cells using GTX46327 TMEM104 antibody at 5.0microg/ml.
WB analysis of Jurkat cells using GTX46327 TMEM104 antibody at 5.0microg/ml.
WB analysis of Jurkat cells using GTX46327 TMEM104 antibody at 5.0microg/ml.

TMEM104 antibody, Internal

GTX46327
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSLC38A12
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Overview

  • Supplier
    GeneTex
  • Product Name
    TMEM104 antibody, Internal
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID54868
  • Target name
    SLC38A12
  • Target description
    solute carrier family 38 member 12
  • Target synonyms
    TMEM104, transmembrane protein 104
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NE00
  • Protein Name
    Transmembrane protein 104
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMEM104 using anti-TMEM104 antibody (A16095-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM104 antigen affinity purified polyclonal antibody (Catalog # A16095-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM104 at approximately 56 kDa. The expected band size for TMEM104 is at 56 kDa.
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