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Western Blot analysis of HeLa, K562, 4T1 cells using TMEM173 Polyclonal Antibody.
Western Blot analysis of HeLa, K562, 4T1 cells using TMEM173 Polyclonal Antibody.
Western Blot analysis of HeLa, K562, 4T1 cells using TMEM173 Polyclonal Antibody.

TMEM173 Antibody

CSB-PA005903
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetSTING1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TMEM173 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID340061
  • Target name
    STING1
  • Target description
    stimulator of interferon response cGAMP interactor 1
  • Target synonyms
    ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING, stimulator of interferon genes protein, N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner, endoplasmic reticulum IFN stimulator, endoplasmic reticulum interferon stimulator, mitochondrial mediator of IRF3 activation, stimulator of interferon protein, stimulator of interferon response cGAMP interactor-deltaC, stimulator of interferon response cGAMP interactor-deltaN, sting 1, transmembrane protein 173
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86WV6
  • Protein Name
    Stimulator of interferon genes protein
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TMEM173 Polyclonal Antibody, Unconjugated (bs-8335R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. _x000D_ _x000D_
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Figure 1. Western blot analysis of TMEM173 using anti-TMEM173 antibody (PB9513). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM173 antigen affinity purified polyclonal antibody (Catalog # PB9513) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM173 at approximately 42 kDa. The expected band size for TMEM173 is at 42 kDa.
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