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ProductsBack/TMEM199 Antibody - N-terminal region (OAAB17817)

TMEM199 Antibody - N-terminal region (OAAB17817)

OAAB17817
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetTMEM199
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    TMEM199 Antibody - N-terminal region (OAAB17817)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
  • Gene ID147007
  • Target name
    TMEM199
  • Target description
    transmembrane protein 199
  • Target synonyms
    C17orf32; CDG2P; transmembrane protein 199; VMA12; VPH2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMEM199 using anti-TMEM199 antibody (A14686-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human U2OS whole cell lysates, Lane 8: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM199 antigen affinity purified polyclonal antibody (Catalog # A14686-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM199 at approximately 23KD. The expected band size for TMEM199 is at 23KD.
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