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Western Blot analysis of HuvEc cells using TMPRSS3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using TMPRSS3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using TMPRSS3 Polyclonal Antibody

TMPRSS3 Antibody

CSB-PA004306
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTMPRSS3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TMPRSS3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64699
  • Target name
    TMPRSS3
  • Target description
    transmembrane serine protease 3
  • Target synonyms
    DFNB10, DFNB8, ECHOS1, TADG12, transmembrane protease serine 3, serine protease TADG-12, transmembrane protease, serine 3, tumor-associated differentially-expressed gene 12 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57727
  • Protein Name
    Transmembrane protease serine 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMPRSS3 using anti-TMPRSS3 antibody (A04164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS3 antigen affinity purified polyclonal antibody (Catalog # A04164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS3 at approximately 48KD. The expected band size for TMPRSS3 is at 48KD.
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