Western blot using GeneTexs protein-A purified anti-pig TNFa antibody shows detection of recombinant pig TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 1 microg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
Western blot using GeneTexs protein-A purified anti-pig TNFa antibody shows detection of recombinant pig TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 1 microg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
TNF alpha antibody
GTX48753
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityPorcine
TargetTNF
Overview
- SupplierGeneTex
- Product NameTNF alpha antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ELISA: 1:2000-1:2500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID397086
- Target nameTNF
- Target descriptiontumor necrosis factor
- Target synonymscachectin; TNFa; TNF-a; TNF-alpha; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
- HostRabbit
- IsotypeIgG
- Protein IDP23563
- Protein NameTumor necrosis factor
- ReactivityPorcine
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203