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Tnf Antibody

CSB-PA325438XA01RA
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetTnf
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Overview

  • Supplier
    Cusabio
  • Product Name
    Tnf Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24835
  • Target name
    Tnf
  • Target description
    tumor necrosis factor
  • Target synonyms
    RATTNF, TNF-alpha, Tnfa, tumor necrosis factor, cachectin, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor ligand superfamily member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16599
  • Protein Name
    Tumor necrosis factor
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (PB9010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PC-12 Whole Cell Lysate, Lane 2: Rat Spleen Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: Rat Kidney Tissue Lysate, Lane 5: Rat Liver Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PB9010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 26KD. The expected band size for TNF alpha is at 26KD.
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