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ProductsBack/TNF Like Ligand 1A (TL1A) Polyclonal Antibody

TNF Like Ligand 1A (TL1A) Polyclonal Antibody

CAU24064
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTNFSF15
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Overview

  • Supplier
    Biomatik
  • Product Name
    TNF Like Ligand 1A (TL1A) Polyclona
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9966
  • Target name
    TNFSF15
  • Target description
    TNF superfamily member 15
  • Target synonyms
    TL1; TL1A; TNF ligand-related molecule 1; TNF superfamily ligand TL1A; TNLG1B; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand 1B; tumor necrosis factor ligand superfamily member 15; tumor necrosis factor superfamily member 15; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A; VEGI; VEGI192A
  • Host
    Rabbit
  • Protein IDO95150
  • Protein Name
    Tumor necrosis factor ligand superfamily member 15
  • Scientific Description
    The TNF Like Ligand 1A (TL1A) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TL1A using anti-TL1A antibody (A02402-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TL1A antigen affinity purified polyclonal antibody (Catalog # A02402-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TL1A at approximately 28KD. The expected band size for TL1A is at 28KD.
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