TNFSF11 / RANKL / TRANCE Antibody (clone R001, Preservative Free)

LifeSpan BioSciences

TNFSF11 / RANKL / TRANCE Antibody (clone R001, Preservative Free)

14

Block: In a functional ELISA which immobilized recombinant Human TNFRSF11A at 2ug/ml (100 microL/well) in the plate, the Rabbit anti-Human TNFSF11 Monoclonal Antibody can block the binding of 0.2 ug/ml of recombinant Human TNFSF11 to human TNFRSF11A, the EC50 is 0.48 ug/ml; Immobilized recombinant Human recombinant Human TNFRSF11B at 2 ug/ml in the plate, the Antibody can block the binding of 0.2 ug/ml of recombinant Human TNFSF11 to human TNFRSF11B, the EC50 is 1.02 ug/ml. Neutralization: The neutralization activity of TNFSF11 Neutralizing Antibody is Measured by its ability to neutralize TNFSF11 induced osteoclast-like cell formation in RAW 264. 7 mouse macrophages. The Neutralization titer (IC50) is typically 4-16 ng/mL in the presence of 30 ng/mL Recombinant Human RANKL/OPGL/TNFSF11/CD254. Please Note: Optimal concentrations/dilutions should be determined by the end user.. Block (0.2 µg/ml), ELISA (2 µg/ml), Neut (30 ng/ml) Block: In a functional ELISA which immobilized recombinant Human TNFRSF11A at 2ug/ml (100 µL/well) in the plate, the Rabbit anti-Human TNFSF11 Monoclonal Antibody can block the binding of 0.2 ug/ml of recombinant Human TNFSF11 to human TNFRSF11A, the EC50 is 0.48 ug/ml; Immobilized recombinant Human recombinant Human TNFRSF11B at 2 ug/ml in the plate, the Antibody can block the binding of 0.2 ug/ml of recombinant Human TNFSF11 to human TNFRSF11B, the EC50 is 1.02 ug/ml. Neutralization: The neutralization activity of TNFSF11 Neutralizing Antibody is Measured by its ability to neutralize TNFSF11 induced osteoclast-like cell formation in RAW 264. 7 mouse macrophages. The Neutralization titer (IC50) is typically 4-16 ng/mL in the presence of 30 ng/mL Recombinant Human RANKL/OPGL/TNFSF11/CD254. Please Note: Optimal concentrations/dilutions should be determined by the end user.

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

R001

Unconjugated

...

TNFSF11

TNF superfamily member 11

CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf, tumor necrosis factor ligand superfamily member 11, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nuclear factor kappa B ligand, tumor necrosis factor (ligand) superfamily, member 11, tumor necrosis factor ligand 6B, tumor necrosis factor superfamily member 11

Rabbit

IgG

-20°C or -80°C,2°C to 8°C

12352203