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Tnip1 Polyclonal Antibody

CAC11325
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNIP1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Tnip1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10318
  • Target name
    TNIP1
  • Target description
    TNFAIP3 interacting protein 1
  • Target synonyms
    ABIN-1, NAF1, VAN, nip40-1, TNFAIP3-interacting protein 1, A20-binding inhibitor of NF-kappa-B activation 1, HIV-1 Nef-interacting protein, Nef-associated factor 1 SNP, virion-associated nuclear shuttling protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15025
  • Protein Name
    TNFAIP3-interacting protein 1
  • Scientific Description
    The Tnip1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity Supplier
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNIP1 using anti-TNIP1 antibody (A02299-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: rat spleen tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNIP1 antigen affinity purified polyclonal antibody (Catalog # A02299-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNIP1 at approximately 80-85KD. The expected band size for TNIP1 is at 72KD.
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